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KMID : 0364819860240020106
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Korean Journal of Microbiology
1986 Volume.24 No. 2 p.106 ~ p.112
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Isolation of Salicylate-Degrading Plasmid from Pseudomonas putida
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Bae, K.S/배경숙
Na, J.U/Kang, S.O/Hong, S.W/Hah, Y.C/Lee, K.J/나종욱/강사욱/홍순우/하영칠/이계준
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Abstract
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1
The large plasmid (about 180 megadaltons) was isolated from the aquatic strain of Pseudomoxas which was found to degrade salicylate. It was found that the plasmid could be isolated under gentle conditions in comparison with other methods. The yield of covalently closed circular DNA was enhanced by heat treatment at 55°C after denaturing the chromosomal DNA with aikaline sodium dodecyl sulfate (pH 12.45), and the plasmid DNA was selectively concentrated by utilizing 10% polyethylene glycol as final concentration. It was also found that the cured strains with mitomycin C did not show any growth on the medium containing salicylate, therefore, it was concluded that the plasmid might play an important role on the salicylate degradation.
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